Tannins from Sanguisorba Officinalis Affect in Vitro Rumen Methane Production and Fermentation
نویسندگان
چکیده
The present study was conducted to evaluate the effect of different doses of the Sanguisorba officinalis tannin extract (SOTE) on in vitro methane production, rumen microorganisms and basic rumen fermentation parameters. SOTE contained 451 mg g-1 of total tannins was tested at different doses (0, 1, 4, 10, 20, 40 and 100 mg of SOTE per vessel; the input of tannins was 0, 1.3, 5.1, 12.8, 25.6, 51.2, 128.0 g per kg dry matter, respectively) during 24 h fermentation of rumen fluid in batch culture system. The basal substrate consisted of meadow hay and barley meal (60:40 ratio). The rumen fluid was collected, pooled and mixed from three ruminally fistulated, Polish Holstein-Friesian dairy cows. The methane production was decreased significantly at the high doses (40 and 100 mg of SOTE per bottle). This favorable decrease at the highest dose (100 mg) was associated with the reduction of in vitro dry matter digestibility (IVDMD), Entodiniomorpha sp. and bacteria populations. The population of methanogens was not affected, as compared with the control treatment. These findings suggest that the highest dose of SOTE has a potential to reduce methane production by decreasing the protozoal population and IVDMD. However, the obtained data clearly showed the stimulating effect of the highest dose of tannins on ammonia release, 40 mg of SOTE should be used in further long-term studies. This dose indicated either antimethanogenic effect even when expressed per units of total gas production or volatile fatty acids. 40 mg of SOTE had no negative impact on in vitro rumen microbial populations, dry matter digestibility and volatile fatty acid content.
منابع مشابه
Bloat Mitigation Potential of Plant Tannins and Yucca Extracts based on in Vitro Ruminal Fermentation and Methane Gas Production from Wheat Forage
Three commercial plant tannin and a yucca extract having different secondary compound profiles were simultaneously evaluated in vitro for their effects on ruminal methane gas production and rumen fermentation patterns of wheat forage. Overall objective was to quantify in vitro, the sources of tannins and dose levels of tannins and yucca extract on rate of gas production, ruminal fermentation, f...
متن کاملEvaluating in vitro dose-response effects of Lavandula officinalis essential oil on rumen fermentation characteristics, methane production and ruminal acidosis
Four in vitro experiments (Exp.) were conducted to evaluate lavender essential oil (LEO) effects at 0 (control), 250 (low dose), 500 (medium dose), 750 and 1000 µL per L(high doses) of incubation medium on rumen gas production kinetics (Exp.1), ruminal digestibility and fermentation (Exp.2), methane production (Exp.3) and rumen acidosis (Exp.4). The asymptote of gas production (A) incr...
متن کاملThe Effect of Native Grass Substitution Using Jengkol (Archidendron jiringa) Peel and Leaves Powder on in vitro Rumen Fermentation
The effect of substituting native grass with jengkol (Archidendron jiringa) by-product on fermentation characteristics, rumen microbial profile, methane production, and hydrogen balance using in vitro method was investigated. Seven treatments (different composition of native grass, jengkol peel, jengkol leaves, and concentrate) with five replications in a block randomized desi...
متن کاملEffects of Selected Medicinal Plants on Rumen Fermentation in a High-concentrate Diet in Vitro
The objective of this in vitro study was to compare fermentation patterns of seven selected traditional medicinal plants and investigate the effects of medicinal plant mixture (MPM) supplements and a high-concentrate diet (lucerne hay and barley grain, LH+BG, 40:60) on rumen fermentation and fatty acid composition. An MPM of Taraxacum officinale L., Acorus calamus L., Calendula officinalis L., ...
متن کاملEffect of rumen bacteria from sheep adapted to a tanninferous diet on in vitro fermentation parameters of pistachio hulls using bovine inoculum
Sheep adapted to consume tannins rich feeds such as oak leaf (OL) appear to develop defensive mechanisms by their ruminal bacteria against these polyphenols. The capabilities of ruminal isolated tannins resistant bacteria from these animals to ferment a tanniniferous feed (i.e., pistachio hulls, (PH) which were incubated with rumen fluid from Holstein dairy cows was assessed. Six g positive coc...
متن کامل